Details of the ultrastructure of Rickettsia prowazekii grown in the chick yolk sac.
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چکیده
منابع مشابه
Characterization of the Madrid E strain of Rickettsia prowazekii purified by renografin density gradient centrifugation.
The avirulent Madrid E strain of Rickettsia prowazekii cultivated in chicken yolk sacs could be purified successfully with a Renografin density gradient method developed previously for Rickettsia typhi. Recovery during purification, viability, and lack of contamination with host cell components were similar for the two species, although yields of R. prowazekii per yolk sac were lower. Purified ...
متن کاملSensitive enzyme-linked immunosorbent assay for detection of antibodies against typhus rickettsiae, Rickettsia prowazekii and Rickettsia typhi.
An enzyme-linked immunosorbent assay (ELISA) has been developed for the titration of rickettsial antibodies in human and animal sera. Two preparations of soluble typhus-group antigens were obtained from Rickettsia typhi and Rickettsia prowazekii by ether extraction: a standard antigen from infected yolk sacs (YS antigen) and one free of yolk sac contaminants from Renografin-purified rickettsiae...
متن کاملThe Rickettsia prowazekii ExoU homologue possesses phospholipase A1 (PLA1), PLA2, and lyso-PLA2 activities and can function in the absence of any eukaryotic cofactors in vitro.
Here we have characterized the Rickettsia prowazekii RP534 protein, a homologue of the Pseudomonas aeruginosa ExoU phospholipase A (PLA) secreted cytotoxin. Our studies showed that purified recombinant RP534 PLA possessed the predicted PLA(2) and lyso-PLA(2) activities based on what has been published for P. aeruginosa ExoU. RP534 also displayed PLA(1) activity under the conditions tested, wher...
متن کاملBiological properties of Rickettsia prowazekii strains isolated from flying squirrels.
Four strains of Rickettsia prowazekii, isolated from flying squirrels (Glaucomys volans volans) from Florida and Virginia, were compared with other strains of the typhus biotype, two previously established strains each of R. prowazekii and R. typhi and one strain of R. canada, for similarities in a number of unrelated phenotypic characteristics. R. akari served as a spotted fever biotype contro...
متن کاملStudies on the Composition of Rickettsia Prowazeki”
The study presented here was undertaken at the request of Colonel H. Plots following observations on the distribution of rickettsial antigens made in the laboratory of the Division of Virus and Rickettsial Diseases, Army Medical School, Washington, D. C. The primary objective was the collection of chemical information on the presence and properties of specific substances in different fractions ...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 94 1 شماره
صفحات -
تاریخ انتشار 1967